Primer

Part:BBa_K5007016

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)


pRS425s R-primer

Reverse primer for amplifying pRS425s - our similar of pRS425, constructed from pRS426 - and pRS426, around the Eco53KI restriction site, adding homology regions for cloning part BBa_K5007008 through Gibson Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
t_m71°C